Interleukin 13 Receptor Alpha 2 (IL 13 Rα2) ELISA Kit, human Assays and Kits

This human Interleukin 13 Rα2 (IL-13 Rα2) ELISA kit assay applies the quantitative sandwich enzyme immunoassay technique. Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. Thus, although IL-13 can induce immunoglobulin E (IgE) secretion from activated human B cells, deletion of IL-13 from mice does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. In comparison, deletion of IL-4 abrogates these responses. Thus, rather than a lymphoid cytokine, IL-13 acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.

A monoclonal antibody specific to IL-13 Rα2 has been pre-coated onto a microplate. Standards and samples provided by this human IL-13 Rα2 ELISA kit are then added to the appropriate plate wells with a biotin -conjugated polyclonal antibody preparation specific for IL-13 Rα2 and incubated. IL-13 Rα2 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. After washing away any unbound substances. In order to quantitatively determine the amount of IL-13 Rα2 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13 Rα2, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The colour development is stopped and the intensity of the colour is measured.

In order to measure the concentration of IL-13 Rα2 in the samples, this human IL-13 Rα2 ELISA kit package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to this human IL-13 Rα2 ELISA kit system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 Rα2 concentration (ng/mL). The concentration of IL-13 Rα2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

This human IL-13 Rα2 ELISA kit is to be used for the in vitro quantitative determination of human interleukin 13 Rα2 concentrations in serum, plasma, cell culture supernatant. This human IL-13 Rα2 ELISA kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in diagnostic or therapeutic procedures.